Table 2.

Testing of primers’ sequences for positive and negative controls DNA samples for leishmaniasis species

Positive/negative control DNA samples	Source	L. major assays	L. infantum /donovani assays	L. mexicana assays	L. viannia assays	L. tropica assays	β-actin assays
L. major DNA	Culture	Positive	Negative	Negative	Negative	Negative	Positive
L. tropica DNA	Culture	Negative	Negative	Negative	Negative	Positive	Positive
L. infantum DNA	Culture	Negative	Positive	Negative	Negative	Negative	Positive
L. donovani DNA	Culture	Negative	Positive	Negative	Negative	Negative	Positive
L. mexicana DNA	Culture	Negative	Negative	Positive	Negative	Negative	Positive
L. (V). braziliens DNA	Culture	Negative	Negative	Negative	Positive	Negative	Positive
Vitiligo DNA	Skin	Negative	Negative	Negative	Negative	Negative	Positive
Psoriasis DNA	Skin	Negative	Negative	Negative	Negative	Negative	Positive
Basal cell carcinoma DNA	Skin	Negative	Negative	Negative	Negative	Negative	Positive
Vitiligo blood DNA	Blood	Negative	Negative	Negative	Negative	Negative	Positive
Psoriasis blood DNA	Blood	Negative	Negative	Negative	Negative	Negative	Positive
Acne DNA	Blood	Negative	Negative	Negative	Negative	Negative	Positive
Atopic dermatitis DNA	Blood	Negative	Negative	Negative	Negative	Negative	Positive
SLE DNA	Blood	Negative	Negative	Negative	Negative	Negative	Positive
Normal DNA	Blood	Negative	Negative	Negative	Negative	Negative	Positive