Table 1.
The primers were used to construct the various truncated, internal deletion mutants by PCR
| Primer name | Type | Length | Sequence (5′-3′) |
|---|---|---|---|
| VP1 EcoR1 | Forward | 25-mer | AGAATTCATGGCAAGACGAGCTCGC |
| VP1 Xho1 R | Reverse | 20-mer | TCCTCGAGGGGCTGCGTCTC |
| VP1 ND9 EcoR1 | Forward | 24-mer | AGAATTCATGGGCCGATTTTACGC |
| VP1 DN19 EcoR1 | Forward | 26-mer | AGAATTCATGCACAACCTCAAGCGAC |
| VP1 ND30 EcoR1 | Forward | 25-mer | AGAATTCAAATTTCGCCATCGCCGC |
| VP1 ND60 EcoR1 | Forward | 25-mer | AGAATTCCTGCCGAACCCGCAGAGC |
| VP1 ND129 EcoR1 | Forward | 25-mer | AGAATTCGGCGAACTGATTGCGGAT |
| VP1 CD10 Xho1 | Reverse | 21-mer | TCCTCGAGGCCTCTCGGTCTG |
| VP1 CD103 Xho1 | Reverse | 24-mer | TCCTCGAGGGCGACTCTCGGCCCC TAAGATGG |
| VP1 CD126 Xho1 | Reverse | 22-mer | TCTCGAGTCCGATTTTGCTCAC |
| VP1 internal NES deletion 1162 | Forward | 24-mer | GCGGCCGCGGCACAAATAAGTCGC |
| VP1 internal NES deletion 1119 | Reverse | 27-mer | GCGGCCGCATGATGCGGGTGCACT |