Table 1.

The description of candidate reference genes and primers used in this study

Gene symbol	Gene name	Gene ID	Arabidopsis homolog	Forward primer sequence (5′–3′)	Reverse primer sequence (5′–3′)	Size (bp)	E (%)	R2
ACT7	Actin 7	Potri.001G309500	AT5G09810	GCATCCACGAGACTACATACAACTCA	GTGATCTCCTTGCTCATTCGGTCA	136	100.02	0.998
EIF4A	Eukaryotic initiation factor 4A	Potri.005G093900	AT3G19760	TACATTCATCGAATTGGTCGTTCTGGT	TTCATAGGCATTTCGTCAATCTGGG	137	101.50	0.995
GAPDH	Glyceraldehyde-3-phosphate dehydrogenase	Potri.012G094100	AT1G13440	AACCGACTTCATTGGTGACAACCG	CCACTCATTGTCATACCACGCAAC	106	100.72	0.997
Histone	Histone superfamily protein	Potri.005G072300	AT4G40030	ACTGTTGCTCTTCGTGAAATCCGTA	CTTAAAATCCTGGGCAATTTCACGAAC	105	96.62	0.998
PP2A-2	Protein phosphatase 2A-2	Potri.015G068300	AT1G10430	ACAGTTCAACCACACTAATGGGCTC	TTTGGCGCACTGAACACTGTAACCAC	114	103.41	0.988
PP2A-A2	Protein phosphatase 2A subunit A2	Potri.010G127500	AT3G25800	ATGAATTTCCTGATGTGCGACT	CAATGCCTATCCTCTGCAAGCTC	127	99.23	0.998
RPS18	Ribosomal protein S18	Potri.006G170500	AT1G07210	AGGCTCATCATCTTATCAAATCCCT	TCAATGCCACCAAATATTCGTTGCT	127	103.69	0.990
UBQ10	Polyubiquitin 10	Potri.001G418500	AT4G05320	GTTGATTTTTGCTGGGAAGC	GATCTTGGCCTTCACGTTGT	192	98.18	1.000
ATPase	ATP synthase subunit B	Potri.004G177500	AT4G38510	ACTCATCCCACCCCTGATCTTACGG	ACCAATGGCACTCTTCATGAGACGA	138	101.65	0.995
UBP	Oligouridylate binding protein 1B	Potri.006G279600	AT1G17370	GGCTTTGTTTCATTCCGTAATCAGCA	AACACCTTTAGTTGCCCAATTGCAT	111	100.92	0.978
bHLH	bHLH transcription factor	Potri.011G132400	AT5G54680	ATCTGAATCGTGTAGTGCGTCTAGCTC	GCATCAACCAAAATAGCAGCCTTGTCC	147	101.61	0.985
DNAJ	DNAJ homologue 2	Potri.010G243100	AT5G22060	AGGCAATTAATGACAAGGACCGTT	AGCCTCTCCAGGGAAAGTAATCCTC	131	101.98	0.996
U6-1	Small nuclear ribonucleoprotein family protein	Potri.001G166600	AT3G14080	GTGACCTTTATTGCGACATCCACT	CTTCTGAAACACGAGTCATATGTGGT	123	96.16	0.995
U6-2	Small nuclear ribonucleoprotein family protein	Potri.008G078400	AT2G43810	GCCTGTTGTGGTTAAGCTCAATTCTG	TTCCTCGTATGAAAGCATCACCAT	149	99.12	0.999
5.8s	5.8S ribosomal RNA gene	AJ006440		ACGTCTGCCTGGGTGTCAC	TCAACCACCGCTCGTCGTG	145	108.37	0.993
miR171	MIMAT0001985	ptc-miR171c		AGATTGAGCCGCGCCAATATC	AACGAGACGACGACAGACTTT	49	107.44	0.967
miR403	MIMAT0002056	ptc-miR403a		CGCGTTAGATTCACGCACAAACTC	AACGAGACGACGACAGACTTT	57	115.04	0.982
miR482	MIMAT0002103	ptc-miR482a.1		CCTACTCCTCCCATTCCAAAA	AACGAGACGACGACAGACTTT	50	116.69	0.941