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. 2019 Apr 6;15:35. doi: 10.1186/s13007-019-0420-1

Table 3.

Ranking of the expression stability of candidate reference genes calculated by BestKeeper during plant regeneration

Rank Callus Seedlings Plants Total
Gene SD CV Gene SD CV Gene SD CV Gene SD CV
1 EIF4A 0.07 0.28 Histone 0.27 1.14 EIF4A 0.43 1.63 EIF4A 0.37 1.39
2 U6-2 0.08 0.31 U6-1 0.28 1 U6-2 0.49 1.77 U6-1 0.39 1.38
3 U6-1 0.12 0.41 EIF4A 0.28 1.09 U6-1 0.52 1.83 Histone 0.39 1.65
4 UBQ10 0.15 0.65 U6-2 0.29 1.09 Histone 0.53 2.2 U6-2 0.44 1.6
5 Histone 0.16 0.65 RPS18 0.37 1.22 PP2A-2 0.54 1.99 PP2A-2 0.49 1.79
6 ACT7 0.17 0.65 bHLH 0.4 1.48 GAPDH 0.58 2.4 bHLH 0.61 2.21
7 ATPase 0.17 0.63 miR171 0.44 1.91 RPS18 0.6 1.94 GAPDH 0.61 2.54
8 GAPDH 0.19 0.79 GAPDH 0.45 1.89 bHLH 0.69 2.48 UBP 0.66 2.1
9 PP2A-2 0.19 0.69 PP2A-2 0.49 1.82 PP2A-A2 0.71 2.53 PP2A-A2 0.66 2.38
10 PP2A-A2 0.32 1.15 DNAJ 0.49 1.96 DNAJ 0.71 2.65 ACT7 0.7 2.64
11 bHLH 0.33 1.22 ACT7 0.56 2.19 ATPase 0.75 2.74 UBQ10 0.7 3.1
12 5.8S 0.33 1.5 UBQ10 0.57 2.55 UBP 0.76 2.39 DNAJ 0.71 2.69
13 RPS18 0.34 1.15 UBP 0.6 1.94 ACT7 0.81 3.08 ATPase 0.74 2.7
14 DNAJ 0.42 1.62 PP2A-A2 0.6 2.21 UBQ10 0.84 3.74 RPS18 0.76 2.48
15 UBP 0.44 1.4 ATPase 0.63 2.36 5.8S 1.09 5.03 5.8S 0.93 4.25
16 miR482 0.48 2.39 5.8S 0.79 3.62 miR482 1.27 6.11 miR482 1.11 5.39
17 miR403 0.64 2.75 miR403 0.83 3.36 miR171 1.82 7.68 miR171 1.48 6.39
18 miR171 0.88 4.14 miR482 1.22 5.8 miR403 1.97 7.62 miR403 1.63 6.47