Table 4.
Expression stability ranking of the 18 candidate reference genes during plant regeneration
| Method | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 |
|---|---|---|---|---|---|---|---|---|---|
| (A) Ranking order across tissues at callus stage (better–good–average) | |||||||||
| Genorm | U6-1|U6-2 | EIF4A | UBQ10 | GAPDH | ACT7 | PP2A-2 | ATPase | Histone | |
| Normfinder | Histone | EIF4A | U6-2 | U6-1 | PP2A-2 | ACT7 | ATPase | UBQ10 | GAPDH |
| BestKeeper | EIF4A | U6-2 | U6-1 | UBQ10 | Histone | ACT7 | ATPase | GAPDH | PP2A-2 |
| Delta CT | EIF4A | U6-2 | U6-1 | Histone | PP2A-2 | ATPase | ACT7 | UBQ10 | GAPDH |
| RankAggreg | EIF4A | U6-2 | U6-1 | Histone | UBQ10 | ACT7 | PP2A-2 | ATPase | GAPDH |
| (B) Ranking order across tissues at seedlings stage (better–good–average) | |||||||||
| Genorm | U6-1|U6-2 | EIF4A | RPS18 | bHLH | PP2A-2 | ACT7 | GAPDH | UBP | |
| Normfinder | U6-1 | U6-2 | EIF4A | RPS18 | bHLH | GAPDH | ACT7 | PP2A-2 | DNAJ |
| BestKeeper | Histone | U6-1 | EIF4A | U6-2 | RPS18 | bHLH | miR171 | GAPDH | PP2A-2 |
| Delta CT | U6-2 | U6-1 | EIF4A | PP2A-2 | RPS18 | bHLH | ACT7 | GAPDH | UBP |
| RankAggreg | U6-1 | U6-2 | EIF4A | RPS18 | bHLH | PP2A-2 | ACT7 | GAPDH | DNAJ |
| (C) Ranking order across tissues at plants stage (better–good–average) | |||||||||
| Genorm | PP2A-2|U6-1 | RPS18 | U6-2 | EIF4A | bHLH | DNAJ | UBP | GAPDH | |
| Normfinder | UBP | bHLH | DNAJ | U6-1 | PP2A-2 | RPS18 | PP2A-A2 | UBQ10 | EIF4A |
| BestKeeper | EIF4A | U6-2 | U6-1 | Histone | PP2A-2 | GAPDH | RPS18 | bHLH | PP2A-A2 |
| Delta CT | PP2A-2 | U6-1 | bHLH | UBP | DNAJ | RPS18 | PP2A-A2 | EIF4A | GAPDH |
| RankAggreg | U6-1 | PP2A-2 | bHLH | RPS18 | EIF4A | U6-2 | UBP | DNAJ | PP2A-A2 |
| (D) Ranking order under all samples (better–good–average) | |||||||||
| Genorm | EIF4A|U6-1 | U6-2 | PP2A-2 | bHLH | PP2A-A2 | UBP | ATPase | GAPDH | |
| Normfinder | bHLH | PP2A-2 | UBP | U6-1 | U6-2 | EIF4A | GAPDH | PP2A-A2 | DNAJ |
| BestKeeper | EIF4A | U6-1 | Histone | U6-2 | PP2A-2 | bHLH | GAPDH | UBP | PP2A-A2 |
| Delta CT | PP2A-2 | U6-1 | bHLH | U6-2 | UBP | EIF4A | PP2A-A2 | GAPDH | ATPase |
| RankAggreg | U6-1 | EIF4A | PP2A-2 | U6-2 | UBP | PP2A-A2 | bHLH | GAPDH | Histone |
| Method | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 |
|---|---|---|---|---|---|---|---|---|---|
| (A) Ranking order across tissues at callus stage (better–good–average) | |||||||||
| Genorm | PP2A-A2 | RPS18 | bHLH | DNAJ | UBP | 5.8S | miR482 | miR403 | miR171 |
| Normfinder | RPS18 | 5.8S | bHLH | PP2A-A2 | UBP | DNAJ | miR482 | miR403 | miR171 |
| BestKeeper | PP2A-A2 | bHLH | 5.8S | RPS18 | DNAJ | UBP | miR482 | miR403 | miR171 |
| Delta CT | PP2A-A2 | RPS18 | bHLH | 5.8S | DNAJ | UBP | miR482 | miR403 | miR171 |
| RankAggreg | RPS18 | PP2A-A2 | bHLH | 5.8S | DNAJ | UBP | miR482 | miR403 | miR171 |
| (B) Ranking order across tissues at seedlings stage (better–good–average) | |||||||||
| Genorm | DNAJ | PP2A-A2 | ATPase | UBQ10 | Histone | 5.8S | miR171 | miR403 | miR482 |
| Normfinder | UBP | PP2A-A2 | Histone | UBQ10 | ATPase | 5.8S | miR171 | miR403 | miR482 |
| BestKeeper | DNAJ | ACT7 | UBQ10 | UBP | PP2A-A2 | ATPase | 5.8S | miR403 | miR482 |
| Delta CT | DNAJ | PP2A-A2 | ATPase | UBQ10 | Histone | 5.8S | miR171 | miR403 | miR482 |
| RankAggreg | UBP | PP2A-A2 | Histone | UBQ10 | ATPase | 5.8S | miR171 | miR403 | miR482 |
| (C) Ranking order across tissues at plants stage (better–good–average) | |||||||||
| Genorm | PP2A-A2 | ATPase | ACT7 | UBQ10 | Histone | 5.8S | miR171 | miR482 | miR403 |
| Normfinder | Histone | ACT7 | U6-2 | GAPDH | ATPase | 5.8S | miR171 | miR482 | miR403 |
| BestKeeper | DNAJ | ATPase | UBP | ACT7 | UBQ10 | 5.8S | miR482 | miR171 | miR403 |
| Delta CT | U6-2 | ACT7 | UBQ10 | ATPase | Histone | 5.8S | miR171 | miR482 | miR403 |
| RankAggreg | GAPDH | Histone | ACT7 | ATPase | UBQ10 | 5.8S | miR171 | miR482 | miR403 |
| (D) Ranking order under all samples (better–good–average) | |||||||||
| Genorm | ACT7 | DNAJ | Histone | UBQ10 | RPS18 | 5.8S | miR482 | miR171 | miR403 |
| Normfinder | Histone | ATPase | ACT7 | RPS18 | UBQ10 | 5.8S | miR171 | miR482 | miR403 |
| BestKeeper | ACT7 | UBQ10 | DNAJ | ATPase | RPS18 | 5.8S | miR482 | miR171 | miR403 |
| Delta CT | ACT7 | DNAJ | Histone | UBQ10 | RPS18 | 5.8S | miR171 | miR482 | miR403 |
| RankAggreg | ATPase | ACT7 | DNAJ | UBQ10 | RPS18 | 5.8S | miR482 | miR171 | miR403 |