Fig. 5.

Silence of ST6GALNAC1 suppressed the self-renewal ability of OCSCs. a Detection of CD44, Nanog and Oct4 by RT-qPCR; b detection of CD44, Nanog and Oct4 by western blot analysis; c tumorsphere formation assay was used to test the sphere-forming ability; d colony formation in soft agar assay was conducted to examine the colony-forming ability. The measurement data were expressed as mean ± standard deviation. The results mentioned above were analyzed by t-test. *p < 0.05 vs. the si-NC group; ^#p < 0.05 vs. the empty vector group; the experiment was repeated 3 times