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. 2019 Mar 19;8(3):bio041095. doi: 10.1242/bio.041095

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© 2019. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — Different subcellular localizations of transfected epitope-tagged Jag1 constructs. In transfected B3 lens progenitor cells co-labeled with rabbit anti-HA (green) and goat anti-Jag1 (red) antibodies, FL-Jag1-HA is present throughout the cell but excluded from nucleus (A–A″). The distribution of Jag1-CTF-HA is similar to the full length tagged protein except also uniquely displays some nuclear localization (B–B″). J1-ICD-HA transfected cells exhibit strong nuclear expression (C–C″). n=3 biological replicate experiments for each construct. Scale bar: 50 µm.