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. 2019 Mar 15;8(3):bio039842. doi: 10.1242/bio.039842

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© 2019. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — Somatic knockdown of hbs or sns inhibits EC membrane extension. In all panels: Anti-Coracle (Cora, green) marks somatic cell membranes, Anti-Tj marks ECs (magenta). Arrowheads point to existing extensions, insets show region 2a magnified twofold. (A) Control tj-GAL4>lacZ exhibits normal EC extensions. (B) Somatic knockdown of hbs using RNAi exhibits reduced EC extensions. (C) Somatic knockdown of sns using RNAi exhibits reduced EC extensions. (D) Quantification of the percentage of germaria with reduced extensions observed in somatic knockdown of hbs (n=80, six independent experiments) or sns (n=57, four independent experiments). P-values were calculated using binomial proportions z-test, bars represent s.d. Scale bars: 10 µm.