Fig. 4.

rst is required for Egfr activation in ECs. In all panels: Anti-Coracle (Cora, green) marks somatic cell membranes, Anti-dpERK (magenta) marks somatic cell bodies. (A,A′) Control ECs express dpERK. (B,B′) rst homozygous mutant ECs have reduced dpERK levels. Arrowheads indicate ECs. (C) Quantification of dpERK fluorescent levels in ECs of control (OregonR, n=67) or rst (n=60) mutant germaria. Six independent experiments are shown. P-values were calculated using two-sample t-test, bars represent s.d. Scale bars: 10 µm.