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Expression analyses of housekeeping genes prps1 and dicer1 by qPCR. Primer pairs amplifying spliced transcripts (ee, mature RNA) or pre-spliced (de novo) transcripts (ei, precursor RNA) were used. Copy numbers per reaction were calculated and normalized taking the highest amount as 1. RT(−) are control qPCR trials for each primer set using RNA as templates (represented in black). The timing of midblastula transition (MBT) is indicated.
