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. 2019 Mar 29;25:2289–2295. doi: 10.12659/MSM.912392

Figure 2.

Figure 2

SIRT1 overexpression markedly upregulates osteogenic proliferation. SIRT1 overexpression upregulated the protein (A) and mRNA (B) levels and cell proliferation (C) in MC3T3-E1. Cells were either untreated or transfected with empty plasmid transfected with overexpression plasmid of SIRT1. The SIRT1 interference downregulated the protein (D) and mRNA (E) levels and cell proliferation (F) in MC3T3-E1 cells. Cells were either untreated or transfected with empty plasmid transfected with SIRT1 interference plasmid. SIRT1 protein and mRNA levels were measured by Western blotting and PCR, GAPDH was used as the endogenous control, and the cell viability of MC3T3-E1 cells was measured with CCK8 assay, then quantified and statistically analyzed. * P<0.05, ** P<0.01, *** P<0.001 vs. MC3T3-E1 group.