Figure 3.

Effects of stressors on the growth of 87 plasma-sensitive strains. (a) To determine growth defects of the mutants in LB and M9 medium at 37°C, the OD after a 16 h incubation in the respective medium was set in relation to that of the wild-type, for which the growth defect was set to 0%. (b) To determine growth defects of the strains in the presence of stressors, cells were incubated in LB medium containing the indicated plasma-mimicking components. Acid stress was caused by HCl, disulfide stress by diamide, heat stress by overnight incubation at 40.5°C, and membrane stress by addition of different detergents. The OD of the deletion strains after 16 h incubation was set in relation to the corresponding unstressed control and subsequently in relation to the wild-type (set to 0% growth defect). A growth defect greater than 20% was regarded significant. All data represent three independent biological replicates.