Table 1.

Plasma-protective genes. Strains of the KEIO collection were spotted onto LB agar and exposed to the effluent of the µAPPJ for 100 s. The jet was driven with He and a 0.6% O₂ admixture. Genes were listed as protective when the respective knockout mutant did not form colonies after plasma exposure and a 16 h incubation in at least two out of three experiments. Annotations are based on NCBI database.

#	gene	protein	NCBI ID	#	gene	protein	NCBI ID
1	abgT	p-aminobenzoyl glutamate:H+ symporter	945912	29	holC	DNA polymerase III, χ subunit	948787
2	ada	DNA-binding transcriptional regulator	946710	30	holD	DNA polymerase III, ψ subunit	948890
3	apaH	diadenosine tetraphosphatase	944770	31	ihfA	integration host factor, α subunit	945472
4	asnB	asparagine synthetase B	945281	32	ihfB	integration host factor, β subunit	945533
5	atoA	β subunit, acetate CoA transferase	946719	33	iscS	cysteine desulfurase	947004
6	atoE	short chain fatty acid transporter	946721	34	iscU	scaffold protein for iron–sulfur cluster assembly	947002
7	bamB	outer membrane protein assembly complex	946982	35	katE	catalase II	946234
8	bcsC	cellulose biosynthesis protein	948047	36	lpd	lipoamide dehydrogenase	944854
9	carA	carbamoyl phosphate synthetase	949025	37	lsrD	autoinducer-2 ABC transporter	946264
10	cpxA	sensory histidine kinase	948405	38	menC	O-succinylbenzoate synthase	946734
11	csgG	curli secretion channel	945619	39	mglB	d-galactose/d-galactoside ABC transporter	949041
12	cysB	transcriptional regulator	945771	40	mngA	2-O-α-mannosyl-d-glycerate PTS permease	945355
13	degP	serine protease Do	947139	41	mntH	Mn2+/Fe2+:H+ symporter	946899
14	dksA	RNA polymerase-binding transcriptional factor	944850	42	moaC	cyclic pyranopterin monophosphate synthase	945397
15	dnaT	primosomal protein	948813	43	nagC	DNA-binding transcriptional dual regulator	945285
16	ecnB	bacteriolytic entericidin B lipoprotein	2847737	44	napC	periplasmic nitrate reductase	946706
17	essQ	Qin prophage, lysis protein	946093	45	nlpE	outer membrane lipoprotein involved in surface sensing	946782
18	fimC	periplasmic chaperone for type 1 fimbriae	948843	46	oppD	murein tripeptide ABC transporter	945802
19	fimD	export and assembly of type 1 fimbriae	948844	47	oxyR	OxyR DNA-binding transcriptional dual regulator	948462
20	flgB	flagellar basal-body rod protein FlgB	945678	48	pepQ	XAA-Pro dipeptidase	948335
21	flgG	flagellar basal-body rod protein FlgG	945647	49	pgm	phosphoglucomutase	945271
22	ftn	ferritin iron storage protein	946410	50	pnp	polynucleotide phosphorylase	947672
23	fucA	l-fuculose-phosphate aldolase	947282	51	poxA	EF-P-lysine lysyltransferase	7156434
24	gspL	putative protein secretion protein for export	947842	52	priA	primosome factor N'	948426
25	guaA	GMP synthetase	947334	53	purH	AICAR transformylase/IMP cyclohydrolase	948503
26	hdeB	acid stress chaperone	948026	54	rcsC	sensory histidine kinase	948993
27	hicA	toxin of the HicA–HicB toxin–antitoxin system	945989	55	recA	DNA strand exchange and recombination protein	947170
28	hlpA	chaperone protein	944861	56	rep	DNA helicase	948292
57	rimM	ribosome maturation protein	947101	73	ydhQ	conserved protein	944851
58	rnt	RNase T	946159	74	ydhX	predicted 4Fe-4S ferredoxin-type protein	947308
59	rpmJ	50S ribosomal subunit protein L36	947805	75	ydiL	conserved protein	946181
60	rsxA	integral membrane protein of SoxR-reducing complex	946148	76	ydjA	predicted oxidoreductase	945964
61	rsxC	member of SoxR-reducing complex	946137	77	yeiA	NADH-dependent dihydropyrimidine dehydrogenase subunit	949037
62	sufB	SufBCD iron–sulfur cluster scaffold protein	945753	78	yeiG	S-formylglutathione hydrolase/S-lactoylglutathione hydrolase	949045
63	thyA	thymidylate synthase	949035	79	yfaU	2-keto-3deoxy-l-rhamnoate aldolase	948054
64	trpB	tryptophan synthase, β subunit	945768	80	ygjR	predicted NAD(P)-binding dehydrogenase	947600
65	tufA	elongation factor Tu	947838	81	yifL	predicted lipoprotein	1450304
66	wzxE	lipid III flippase	948294	82	yigA	conserved protein	948359
67	yaiT	putative protein	4056040	83	ymgG	putative protein	945728
68	ybcS	DLP12 prophage, lysozyme	7156271	84	yncK	predicted transposase	4056028
69	ybdO	predicted DNA-binding transcriptional regulator	945216	85	yojL	flavin transferase	946711
70	ycjU	β-phosphoglucomutase	945891	86	yqfA	predicted oxidoreductase	947381
71	ydeS	predicted fimbrial-like adhesin protein	946047	87	zapA	cell division factor	947404
72	ydfH	DNA-binding transcriptional regulator	7158646