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. 2019 Mar 18;222(6):jeb188300. doi: 10.1242/jeb.188300

Fig. 5.

Fig. 5.

Opsin and Cyp27c1 expression. (A) Relative cone opsin expression profile of C. monoculus samples normalized to the β-actin gene. (B) Relative cone opsin expression of C. monoculus based on FPKM values (fragments per kilobase of transcript per million reads). (C) Normalized opsin expression as total FPKM values. (D) Opsin expression of seven C. monoculus samples. Opsin expression values were normalized to the β-actin gene. Each opsin class is specified (colored circles). (E) Gene expression of Cyp27c1 based on FPKM counts and (F) Cyp27c1 expression normalized to β-actin gene (gray circles). Dashed line separates samples from different sites (clear versus turbid). Light circles denote samples from clear waters whereas dark circles denote samples from turbid water sites. Black borders denote duplicates from a single fish and bigger circles are used to reveal overlapping data points.