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. 2019 Mar 4;12(3):dmm037697. doi: 10.1242/dmm.037697

Fig. 1.

Fig. 1.

β-catenin regulates BCL-3 expression in CRC cells. (A) Survival analysis in relation to BCL-3 expression generated using a publicly available CRC dataset (GSE24551) and Progene V2 (Goswami and Nakshatri, 2014). (B) Western blot analysis of adenoma- and carcinoma-derived colorectal cell lines showing expression of BCL-3 and β-catenin. α-tubulin serves as a loading control. (C) Western analysis of total and active β-catenin and BCL-3 expression in LS174T cells with dox-inducible expression of β-catenin shRNA following 24, 48 and 72 h of dox treatment (1 µg/ml). LS174T/R1 cells possess a dox-responsive promoter upstream of a scrambled shRNA sequence and express a non-targeted shRNA upon treatment with dox. α-tubulin serves as a loading control. (D) Western analysis of β-catenin and BCL-3 expression in LS174T cells at 24, 48 and 72 h post-β-catenin siRNA transfection (25 nM). β-catenin siSTABLE is a β-catenin-targeted siRNA with enhanced stability. α-tubulin serves as loading control. Dox, doxycycline.