Table 2.
Parameters validating the method of analysis of glibenclamide
| Parameter | Suspension A | Suspension B |
|---|---|---|
| Linear range (µg/mL) | 50.0–150.0 (y=2175.5x−7699) |
50.0–150.0 (y=2127x−7615) |
| R2 | 0.9927 | 0.9951 |
| LOD (µg/mL) | 0.19 | 0.23 |
| LOQ (µg/mL) | 0.62 | 0.74 |
| Precision (% RSD) | ||
| Intraday (n=6) | ||
| Retention time | 0.1 | 0.1 |
| Peak area | 0.8 | 1.0 |
| Interday (n=18) | ||
| Retention time | 0.2 | 0.4 |
| Peak area | 2.5 | 2.4 |
| Accuracy | ||
| Spiked levels | ||
| 80% | 100.7 (RSD=1.3) | 106.0 (RSD=1.8) |
| 100% | 99.2 (RSD=0.8) | 103.7 (RSD=1.1) |
| 120% | 102.5 (RSD=1.9) | 105.9 (RSD=2.3) |
LOD, limits of detection; LOQ, limits of detection quantification; RSD, relative standard deviation.