Table 3.

Patient specific allelic discrimination Q-PCR assay information

Gene Reference	Mutation	Patient	Probe-wild type seq-VIC lablelled	Probe-mutant seq-FAM lablelled	Forward primer	Reverse primer
CBL	p.G413D	Patient 4	AGGAATCAGAAGGTCAG	AGGAATCAGAAGATCAG	TGCATCTGTTACTATCTTTTGCTTCTTC	ATTTCACATCGGCAGAAAGGA
DNMT3A	p.R882C	Patient 1	CCAAGCGGCTCAT	CCAAGCAGCTCAT	CCGGCCCAGCAGTCTCT	CAGTCCACTATACTGACGTCTCCAA
DNMT3A	p.M682fs*23	Patient 4	N/A	CGACGTACATATCTTC	CCCCACAGCATGGACATACA	CATCACGGTGGGCATGGT
DNMT3A	p.R882H	Patient 5, 7, 8, 10	CCAAGCGGCTCAT	CCAAGTGGCTCATG	CCGGCCCAGCAGTCTCT	TGGTTTCCCAGTCCACTATACTGA
FLT3	p.D835E	Patient 7	ACTCATGATATCTCG	TCACTCATGATCTCTCGA	GCCCCTGACAACATAGTTGGA	GTGGTGAAGATATGTGACTTTGGATT
FLT3	p.M664I	Patient 3	CTGGGTCATCATCT	CTGGGTCATTATCT	CCCCAGCAGGTTCACAATATTC	AAGAGAGGCACTCATGTCAGAACTC
FLT3	p.N841K	Patient 3	CTGACAACATAGTTGGAA	CTGACAACATATTTGG	AAATAAGTAGGAAATAGCAGCCTCACA	GGATTGGCTCGAGATATCATGAGT
GATA1	p.P38L	Patient 2	CCTCTGGGCCTGAG	TGGGCTTGAGGGC	GTGTCCTCCACACCAGAATCAG	GAGGAAGCTGCTGCATCCA
GATA2	p.N402S	Patient 8	TGGACTTGTTGGACAT	TCTTCTTGGACTTGCTG	TTTGACAGCTCCTCGAAGCA	CAGGCCACTGACCATGAAGA
IDH2	p.R140Q	Patient 2	CCAGGATGTTCCGGAT	CCAGGATGTTCTGGAT	GGGCTCCCGGAAGACAGT	TGTGGAAAAGTCCCAATGGAA
MLL5	p.S556N	Patient 8	AACTCCTATTAGTAATGAAG	AACTCCTATTAATAATGAAG	CATTTTTCAGGAACCAGATTTTATTG	CATCTTCCTTTTCCTTTCTGCAA
NF1	p.S2243fs*14	Patient 5	N/A	ATATAATCCATTCCCTGCAACC	TCTTTTAATTGCAGATTTGCATTCC	GCTAATACACCCAAAGACAACAAGAG
NPM1-B	p.W288fs*12	Patient 1, 5, 9	N/A	TTCCAGGCTATTCAAG	ATGTCTATGAAGTGTTGTGGTTCCTT	TCCTCCACTGCCAGACAGAGA
NPM1-A	p.W288fs*12	Patient 3, 4, 6, 7, 8, 10	N/A	AAGATCTCTGTCTGGCAGTG	TGTCTATGAAGTGTTGTGGTTCCTTAA	CTGTTACAGAAATGAAATAAGACGGAAA
NPM1-D	p.W288fs*12	Patient 2	N/A	TTCAAGATCTCTGCCTGGC	TGTCTATGAAGTGTTGTGGTTCCTTAA	CTGTTACAGAAATGAAATAAGACGGAAA
NRAS	p.G13D	Patient 2	CCAACACCACCTGC	CCAACATCACCTGCT	CTGGATTGTCAGTGCGCTTTT	TTGCTGGTGTGAAATGACTGAGT
PTPN11	p.E76G	Patient 5	CCACTTTGGCTGAGT	CCACTTTGGCTGGGTT	CACCCACATCAAGATTCAGAACAC	CCCGTGATGTTCCATGTAATACTG
TET2	p.L1469fs*9	Patient 7	N/A	CGACAAAGGAAAACTA	TGTTAGCAGAGCCAGTCAAGACTT	TCCAGGGAGGAAAGCTTTTCA
TET2	p.Q1624*	Patient 7	TTTGAATCAGAATACCCAAT	TGGGCTTTTGAATTAGAATA	CTTCTAATCCCATGAACCCTTACC	CCACTGATAGGTTTCCATTGCA
TET2	p.R544*	Patient 1	CTGAAGGGTCGAGACAA	CTGAAGGGTTGAGACA	GCCAGCAGTTGATGAGAAACAA	GGCACAAGATCTCGTGTTTGC
TET2	p.S1369*	Patient 4	CCGTCCATTCTCAGG	CCGTCCATTCTGAGG	GCCGTCTGGGTCTGAAGGA	ACAGAAGTCCAAACATGCAGTGA
TET2	p.V1417F	Patient 5, 8	CAGCTTCACGTTCTG	AGCTTCACTTTCTGCCT	TGGAGGAAAACCTGAGGATGA	GAGCTTCCACACTCCCAAACTC
TET2	p.C1374Y	Patient 6	TCTCAAGGAAACCCCAG	TCTCAAGGAAACGCCAG	CAAAAATGTTTGCTCAGGACACA	TCGTGAACCCAACTCTTCTAACTG
TP53	p.R248Q	Patient 10	ATGGGCCTCCGGTT	ATGGGCCTCTGGTT	GGCTCCTGACCTGGAGTCTTC	TGACTGTACCACCATCCACTACAA
WT1	p.A382fs*4	Patient 9	N/A	AGATGCCGACCGACC	GCCTGGTAAGCACACATGA	TGGAGTAGCCCCGACTCTTG
WT1	p.Y402	Patient 2	ACAGCTTAAAATATCTC	ACAGCTTAAACTATCTC	TCCTGCTGTGCATCTGTAAGTG	TGCTTACCCAGGCTGCAATAA
WT1	p.L349fs*26	Patient 3	N/A	CGCAGAGATGGGC	CCGTGCGTGTGTATTCTGTATTG	ACAGGGTACGAGAGCGATAACC
ZRSR2	p.Y274*	Patient 5	TGTATATGTTCAGTACCAGTC	CAATGTATATGTTCAGTAACA	CTAGGTCAGCTGCAATTTGGAA	ACAAATCAGGAAGACACAAG