Figure 2.

High influx of glucose and glutamine in lung SCC detected by PET imaging.

(A) Representative PET and CT images of SCC tumors from KL mice imaged with ¹⁸F-FDG and ¹¹C-Glutamine. (B) Image of whole lungs and heart from mouse imaged in (A). Tumor is circled. T = tumor. H = heart. (C) Left lung lobe and tumor from mouse imaged in (A) stained for hematoxylin and eosin (H&E). Magnified image shown in the inset; scale bar = 25 μm. (D) Serial sections of the left lung lobe stained with CK5/TTF1, GLUT1, SLC1A5 from left lobe from mouse imaged in (A). Magnified images shown in the inset; scale bar = 25 μm. (E) PET/CT images of KL mouse with both ADC and SCC tumors imaged with CT (left), ¹⁸F-FDG (middle), and ¹¹C-Glutamine (right). H = heart, L = liver. T1 and T2 are outlined with dashed lines (T1 = the blue line, T2 = the red line). (F) PET/CT imaged ADC and SCC lung tumors from (E) were stained with antibodies against CK5/TTF1, GLUT1 and SLC1A5 (left side). Scale bar = 1 mm. Magnified images from T1 (blue box) and T2 (red box) are shown on the right side. Scale bar = 50 μm. See also Figure S2.