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. 2019 Mar 21;104(4):731–737. doi: 10.1016/j.ajhg.2019.02.018

Figure 4.

Figure 4

Gene Expression Analysis (RNA Sequencing) of FAM149B1 Mutant Fibroblast Cells Cluster with TBC1D32 Mutant Fibroblast Cells

(A) Correlation matrix showing the similarity between samples of 17DG0722 and 17DG0168 fibroblast cells versus two control subjects are obtained through Pearson’s coefficient of the Log2(FPKM+1) value. For range: −1 ≤ r ≤ 1, the closer the value is to 1, the more similar the samples are.

(B) Heatmap of one-way hierarchical clustering (distance metric = Euclidean distance, linkage method = complete) analysis of DEG (differentially expressed genes) was performed on affected individual 17DG0722 and 17DG0168 fibroblast cells versus two control subjects using FPKM. The result showed 117 genes differentially expressed which satisfied |fc| ≥ 2 and independent t test raw p value < 0.05 conditions. Blue denotes normalized downregulation; yellow denotes upregulation (see attached scale above). The detailed DEG data and the list of the 117 genes are in Table S1.