Figure 3.

Functional Analysis of DLST Variants

(A) A schematic illustration of the tricarboxylic acid (TCA) cycle. Carbon-13 is denoted by green dots in the context of a ¹³C₅ –glutamate labeling experiment. The red arrow pointing at 2-hydroxyglutarate (2HG) represents the collective action of malate and lactate dehydrogenases.

(B) An immunoblot analysis showing equivalent protein levels of wild-type (WT) and mutant DLST in DLST-KO cells.

(C) Labeling patterns of TCA intermediates after ¹³C₅-glutamate labeling.

(D) A pattern of ¹³C₅-2HG labeling after ¹³C₅-glutamate labeling.

(C and D) Error bars represent the SD from a representative experiment. ^∗ p < 0.05 (Student’s t test; n = 3).