Fig. 6.

Influence of the magnetic field on SH-SY5Y cells in 2D culture: specific metabolic activity. a Experimental set-up to evaluate the effect of the magnetic field generated by the ring magnets of the MOAB on SH-SY5Y cells. Scale bar: 5 mm. b Metabolic activity of SH-SY5Y cells with time. For the short exposure time (2 days), we plated 93,750 cells/cm²; while for the long exposure time (7 days), we plated 31,250 cells/cm². TCP indicates SH-SY5Y cells plated on standard tissue culture-treated dishes. c Example of a confocal microscopy image showing live (stained green by calcein AM) and dead (stained red by ethidium homodimer-1) SH-SY5Y cells after 48 h culturing in the absence of the magnetic field. Scale bar: 20 μm. d Specific metabolic activity (that is metabolic activity normalized with respect to the DNA content) of SH-SY5Y cells with time. For the shortest exposure time (2 days), we plated 93,750 cells/cm²; while for the other exposure times, we plated 31,250 cells/cm². In all the graphs, we showed the results from resazurin assay (3 replicates/group). We reported the results as mean ± SD. We performed the statistical analysis with two-way ANOVA followed by Tukey’s multiple comparisons test. ns, p > 0.05; *, p < 0.05; **, p < 0.01; ****, p < 0.0001