Fig. 4.

Plin5 overexpression blunted peroxidation injury induced by NS5A. a HepG2 cells expressing Plin5 (Plin5 OV) and empty vehicle (EV) were established by lentivirus infection. The expression of NS5A and Plin5 in HepG2 cells was analysed by immunoblotting. (b & c) The influence of NS5A on lipid content was observed in HepG2 cells with or without Plin5 overexpression after BODIPY 493/503 staining (b) and TG quantification (c). Scale bar = 10 μm. d Intracellular NEFAs were quantified by commercial kit after extraction with hexane/isopropanol. e HepG2 cells with or without Plin5 overexpression were infected with control (Ad-Null) and NS5A-expressing (Ad-NS5A) adenoviruses. After treatment with 200 μM oleic acid (OA) for 24 h, the ROS levels in HepG2 cells were observed by DCFH-DA staining. Scale bar = 50 μm. f & g The levels of MDA and 4-hydroxynonenal (4-HNE), two lipid oxidation products, were analysed in HepG2 cells with or without NS5A expression. h The triacylglycerol hydrolysis rate in HepG2 cells with or without NS5A expression was determined in the presence of triacsin C (5 μM). *P < 0.05, **P <0.01, ***P < 0.001. Data are means ± SEs for triplicate samples for each representative experiment