Figure 5.

Effects of α- and γ-mangostins on DPPH radical formation and lipid peroxidation (LPO). Inhibition of DPPH radical (a) and LPO induced by Fe²⁺ (10 μM) and L-ascorbic acid (100 μM) in rat forebrain homogenate (b) by α- or γ-mangostin at the indicated concentrations were measured as described in Materials and Methods. Each data point represents the mean ± S.E.M. from at least three independent experiments, performed in duplicate (^∗ P < 0.05 vs. vehicle-treated control without α- or γ-mangostin treatment). Vit. C and BHA were used as references to validate the assay procedures for DPPH radical scavenging activity and inhibition of LPO, respectively (grey bars). Vit. C: vitamin C; BHA: butylhydroxyanisole.