Table 2. Composition of samples for staining of dead cells.

Samplea	Pigment concentration %(W/V)	Pigment [mg]	TBb 1% (W/W) [µL]	MBc 1% (W/W) [µL]	Cell suspension (CM medium) [µL]	BCd 10% (W/V) [µL]
CM	0	0	0	0	196	4
MP	1	2	0	0	196	4
AP	1	2	0	0	196	4
TB (0.05%)	0.05	0.1	0	10	186	4
TB (0.3%)	0.3	0.6	60	0	136	4
MB (0.05%)	0.05	0.1	0	10	186	4
MB (0.3%)	0.3	0.6	60	0	136	4

Notes.

^aCM, Cell Suspension (CM medium); MP, Monascus pigment; AP, Purple sweet potato; TB, Trypan blue (TOKYO CHYEMICAL INDUSTRY Co.,Ltd.); MB, Methylene blue (KOKUSAN CHEMICAL Co., Ltd.).

^bThe solvent of 1% (W/W) TB solution was purified water.

^cThe solvent of 1% (W/W) MB solution was purified water.

^dBC, 10% (W/V) benzalkonium chloride solution (NIHON PHARMACEUTICAL CO., LTD). Since the BC solution is transparent, it does not itself stain cells.