Table 5. Composition of samples for the growth curve and dead cell ratio in the culture mixed with pigment (Figs. 6, 7).
| Sample | Pigment concentration% (W/V) | Pigment[mg] | Cell suspensionb(CM medium) [mL] | Glucose [mg] |
|---|---|---|---|---|
| CM | 0 | 0 | 1 | 0 |
| CM+G | 0 | 0 | 1 | 12 |
| MP | 1 | 10 | 1 | 0 |
| MP+G | 1 | 10 | 1 | 12 |
| AP | 1 | 10 | 1 | 0 |
| AP+G | 1 | 10 | 1 | 12 |
| TB | 0.3 | 3 | 1 | 0 |
| TB+G | 0.3 | 3 | 1 | 12 |
| MB | 0.05 | 0.5a | 0.95 | 0 |
| MB+G | 0.05 | 0.5a | 0.95 | 12 |
Notes.
a
The 0.05 mL of 1% (W/W) methylene blue solution with CM medium as a solvent.
b
“MB” and “MB+G” are 0.05 mL less than other samples. The difference in this amount can be ignored from the result of 5 times of cell density measurement of the following sample.
Sample 1, Cell suspension approximately equal to the initial cell density in this test 6.39 × 105 ± 2.2 × 104 cells/mL (Average ± Standard deviation).
Sample 2, 95% (V/V) of Sample1 diluted with purified water. 6.29 × 105 ± 2.3 × 104 cells/mL (Average ± Standard deviation).