Table 8. Summary of MP and AP for viability assay of E. gracilis revealed.
| Pigment | Color | Concentrationa | Time untilstainingb | Cytotoxicity | Light stability | Expiration date |
|---|---|---|---|---|---|---|
| MP | Red | 0.9∼1.5 % (W/V) | 3 min∼ | Low | Unstable | 4(∼6) days c |
| AP | Red purple | 0.5∼1.0% (W/V) | 3 min∼ | High (without glucose) Low (with glucose) | Stable | 6 days |
Notes.
a
MP, Concentration at which staining of dead cells and health of living cells are compatible.
AP, Concentration at which dead cells are clearly stained and cell health is relatively unaffected.
b
Time of staining of dead cells treated with BC or microwave.
c
Fading appears after 5 days, but staining of dead cells can be visually confirmed up to 6 days.