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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: J Allergy Clin Immunol. 2018 Nov 14;143(4):1536–1548. doi: 10.1016/j.jaci.2018.10.046

Figure 6. M2 stimuli have varying ability to promote macrophage efferocytosis and are independent of functional CFTR.

Figure 6.

(A) Representative gating strategy to quantify efferocytosis of CD14+ macrophages using CFSE+ PMNs. The CFSE+ gate was drawn using control macrophages incubated with unlabeled PMNs. The percentage of CD14+ macrophages positive for ingested apoptotic PMNs (% CFSE+) and quantity of ingested apoptotic PMNs (CFSE MFI) was determined across stimuli. (B) Fold change in percentage of macrophages positive for ingested apoptotic PMNs (PMN) relative to their respective media-only controls. (C) Fold change in CFSE MFI in macrophages relative to their respective media-only controls. *P-value < 0.05 M2 stimuli vs. media control. AZM=azithromycin, MP=methylprednisolone, PMN=apoptotic PMNs, MFI=Mean fluorescence intensity. (Mean ± SEM; n=6/group).