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| Dilution | ×100 | ×500 | ×1,000 |
|---|---|---|---|
| GSE | 3.49 ± 0.02 | 4.45 ± 0.01 | 4.91 ± 0.00 |
| GSE+stop solutiona) | 7.00 ± 0.00 | 7.06 ± 0.00 | 7.04 ± 0.00 |
| Stop solutionb) | 7.07 ± 0.00 | ||
a) 400 µl GSE + 100 µl MM + 500 µl stop solution. b) Stop solution is mixture of 1 M HEPES and FBS (7:3). Data are expressed as mean of three experiments ± standard deviation.
