Table 3. Virucidal and bactericidal efficacies of GSE against viruses and bacteria in aqueous phase.
Pathogen | AIV |
NDV |
IBDV |
SI |
EC |
|||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
FBS | 0% | 5% | 0% | 5% | 0% | 5% | 0% | 5% | 0% | 5% | ||
Positive control | 6.50 ± 0.07a) | 6.75 ± 0.00 | 7.00 ± 0.12 | 6.75 ± 0.00 | 6.84 ± 0.21 | NTb) | 8.30 ± 0.19 | 7.73 ± 0.19 | 7.98 ± 0.25 | 7.23 ± 0.16 | ||
Titer / number of colonies | ×100 | 0 secc) | 4.91 ± 0.35 | 5.00 ± 0.00 | 7.00 ± 0.00 | 6.63 ± 0.06 | 5.62 ± 0.04 | NT | 5.88 ± 0.51 | 7.30 ± 0.00 | 7.15 ± 0.01 | 6.40 ± 0.25 |
5 sec | 3.08 ± 0.17 | 3.91 ± 0.37 | <2.50 ± 0.00 | 3.50 ± 0.00 | 5.61 ± 0.07 | <2.60 ± 0.00 | <2.60 ± 0.00 | <2.60 ± 0.00 | <2.60 ± 0.00 | |||
10 sec | 2.83 ± 0.16 | <2.50 ± 0.00 | <2.50 ± 0.00 | 3.50 ± 0.00 | 5.60 ± 0.10 | <2.60 ± 0.00 | <2.60 ± 0.00 | <2.60 ± 0.00 | <2.60 ± 0.00 | |||
30 sec | 2.83 ± 0.16 | <2.50 ± 0.00 | <2.50 ± 0.00 | 3.50 ± 0.00 | 5.44 ± 0.00 | <2.60 ± 0.00 | <2.60 ± 0.00 | <2.60 ± 0.00 | <2.60 ± 0.00 | |||
×500 | 0 sec | 5.75 ± 0.07 | 5.25 ± 0.00 | 7.00 ± 0.00 | 6.75 ± 0.00 | 5.81 ± 0.21 | NT | 7.41 ± 0.24 | 6.84 ± 0.11 | 7.72 ± 0.09 | 6.73 ± 0.08 | |
5 sec | 4.41 ± 0.24 | 5.66 ± 0.04 | <2.50 ± 0.00 | 6.66 ± 0.04 | 5.70 ± 0.05 | <2.60 ± 0.00 | 3.03 ± 0.20 | <2.60 ± 0.00 | 3.76 ± 0.54 | |||
10 sec | 4.16 ± 0.35 | 5.91 ± 0.14 | <2.50 ± 0.00 | 6.16 ± 0.14 | 5.67 ± 0.07 | <2.60 ± 0.00 | 3.42 ± 0.38 | <2.60 ± 0.00 | 3.53 ± 0.44 | |||
30 sec | 3.16 ± 0.17 | 5.91 ± 0.14 | <2.50 ± 0.00 | 5.33 ± 0.20 | 5.73 ± 0.06 | <2.60 ± 0.00 | <2.60 ± 0.00 | <2.60 ± 0.00 | 2.86 ± 0.07 | |||
×1,000 | 0 sec | 6.00 ± 0.11 | 5.63 ± 0.18 | 6.88 ± 0.06 | 6.50 ± 0.00 | 6.25 ± 0.13 | NT | 7.58 ± 0.28 | 7.24 ± 0.36 | 7.55 ± 0.03 | 6.75 ± 0.07 | |
5 sec | 5.33 ± 0.32 | 5.91 ± 0.04 | <2.50 ± 0.00 | 7.00 ± 0.07 | 5.76 ± 0.06 | <2.60 ± 0.00 | 4.13 ± 0.36 | <2.60 ± 0.00 | 3.99 ± 0.50 | |||
10 sec | 5.41 ± 0.20 | 5.83 ± 0.04 | <2.50 ± 0.00 | 7.00 ± 0.07 | 5.71 ± 0.06 | <2.60 ± 0.00 | 3.09 ± 0.23 | <2.60 ± 0.00 | 3.58 ± 0.46 | |||
30 sec | 5.16 ± 0.39 | 6.16 ± 0.10 | <2.50 ± 0.00 | 7.08 ± 0.10 | 5.73 ± 0.06 | <2.60 ± 0.00 | 2.98 ± 0.18 | <2.60 ± 0.00 | 3.05 ± 0.21 |
a) Virus titer or colony counts are shown in log10, b) Not Tested, c) Reaction time. Data are expressed as the mean of three experiments ± standard deviation.