Table 2. Comparison of sensitivity between LAMP and PCR method using artificial synthetic EEHV 1A POL DNA plasmid.
| Synthetic EEHV 1A POL DNA copies/µl in dilution or blood |
Negative control or non-spiked samples |
50% detection limit (copies/µl) |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 105 | 104 | 103 | 102 | 101 | 10° | 10−1 | ||||
| LAMP assay | ||||||||||
| Dilution with water | 20/20a) | 20/20 | 20/20 | 20/20 | 10/20 | 6/20 | 0/20 | 0/20 | 100.7 | |
| Spiked blood samples | 20/20 | 20/20 | 20/20 | 18/20 | 6/20 | 4/20 | 0/20 | 0/20 | 101.2 | |
| PCR assay | ||||||||||
| Dilution with water | 20/20 | 20/20 | 6/20 | 0/20 | 0/20 | 0/20 | NTb) | 0/20 | 103.3 | |
| Spiked blood samples | 20/20 | 20/20 | 1/20 | 0/20 | 0/20 | 0/20 | NT | 0/20 | 103.5 | |
a) Number of positive samples / number of examined samples. b) NT: Not Tested.