Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Apr 1;10:652. doi: 10.3389/fimmu.2019.00652

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Zeng, Qiu, Chen, Liu, Liu, Liang, Zhang and Dai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Shikonin suppresses T cell proliferation and production of proinflammatory cytokines in vitro. (A) FACS-sorted CD3⁺ T cells from C57BL/6 mice were labeled with CFSE and then co-stimulated with anti-CD3/CD28 Abs and IL-2 in the absence or presence of shikonin or CsA for 4 days. Cell division was measured via FACS analysis. (B) The supernatant above was measured for cytokine production via ELISA. Shikonin suppressed IFN-γ and IL-17A production but increased IL-10 and TGF-β1 levels. Data of column graphs are presented as means ± SD (*P < 0.05 and **P < 0.01, n = 4 wells). One of three separate experiments is shown. (C) Potential cytotoxicity of shikonin on T cells was measured based on cell viability using CCK-8 assays. Data are presented as means ± SD from three independent experiments (*P < 0.05 and **P < 0.01, n = 4 wells).