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. 2019 Mar 4;202(8):2320–2331. doi: 10.4049/jimmunol.1801210

FIGURE 7.

FIGURE 7.

ESS and trimerization domains contribute to the adjuvant effect of Ii-fur for Ab responses. (A and C) Detection of Abs recognizing CIDRα1.1_IT4var19 10 wk after vaccination (2 wk after homologous boost) of BALB/C mice with hAd5–Ii-fur–IT4var19–PFCLINvar30 (n = 5), hAd5–Sp-alb–IT4var19–PFCLINvar30 (n = 5), hAd5–Ii–IT4var19–PFCLINvar30 (n = 4), hAd5–Ii-Cterm-fur–IT4var19–PFCLINvar30 (n = 5), or hAd5–∆17-Ii-fur–IT4var19–PFCLINvar30 (n = 5). Serum was diluted to 1:5 and added to the wells in 2-fold dilutions. (C) Absorbance and dilutions were plotted on a log(x) axis, and AUC were calculated and plotted in (A). (B and D) Detection of Abs recognizing CIDRα1.1_PFCLINvar30 10 wk after vaccination (2 wk postboost) of BALB/C mice with hAd5–Ii-fur–IT4var19–PFCLINvar30 (n = 5), hAd5–Sp-alb–IT4var19–PFCLINvar30 (n = 5), hAd5–Ii–IT4var19–PFCLINvar30 (n = 5), hAd5–Ii-Cterm-fur–IT4var19–PFCLINvar30 (n = 5), or hAd5–∆17-Ii-fur–IT4var19–PFCLINvar30 (n = 5). Serum was diluted to 1:5 and added to the wells in 2-fold dilutions. Absorbance and dilutions were plotted on a log(x) axis (D), and AUC were calculated and plotted in (C). Bars represent the mean of each group with the SD. Each group was compared with the Ii-fur adjuvant group with nonparametric Mann–Whitney U tests. *p < 0.05, **p < 0.001.