Table II. AChR-positive screening result validation by RIA.
Identification/Specimen | Δ% Positive Screening CBA | Δ cpm RIA |
---|---|---|
SLE | 12.5 | 2,469 |
HD (SLE) | 15.5 | −323 |
SLE-neuro 1 | 25.3 | −345 |
SLE-neuro 2 | 15.7 | −20 |
SLE-neuro 3 | 11.4 | −246 |
Positive control CBA | 80.0 | NA |
Negative control CBA | 0.3 | NA |
Positive control RIA | NA | 15,032 |
Negative control RIA | NA | 86 |
Results of validation RIAs (5 μl of serum, whole Ag) are reported. Δ cpm cutoff = 363 (mean + 3 SD).
CBA, cell-based immunofluorescence assay.