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. 2018 Dec 4;28(8):1227–1243. doi: 10.1093/hmg/ddy416

Figure 5.

Figure 5

α-tubulin R402C/H mutants disrupt dynein activity in S. cerevisiae. (A) Time-lapse images of WT, tub1-R403C or tub1-R403H cells labeled with GFP-Tub1, GFP-tub1-R403C or GFP-tub1-R403H, respectively, to illustrate representative dynein sliding events. Dynein sliding is defined by spindle translocation initiated by a microtubule–cortex interaction. (B) Quantification of microtubule–cortical hits in a 10 min period. (C) Percent of microtubule–cortex hits that become dynein sliding events in a 10 min period. (D) Quantification of average sliding distance, measured by spindle translocation during sliding event (μm). At least 34 cells were analyzed for each strain. Data are represented as mean ± SEM. Double asterisks indicate significant difference compared to WT, by t-test (P < 0.01). Quadruple asterisks indicate significant difference compared to WT, by t-test (P < 0.0001). Strains: WT, yJM1887; dyn1∆, 1023; tub1-R403C, yJM2216; tub1-R403H, yJM2218.