Skip to main content
. 2018 Dec 26;28(8):1343–1356. doi: 10.1093/hmg/ddy433

Figure 1.

Figure 1

Generation of conditional Thap1 KO mouse using cre-recombinase with the nestin-promoter. (A) Schematic of KO first gene construct (top) and crossed with either a cre-deleter strain to generate the Thap1 null allele (bottom left) or a Flp-deleter strain to generate the Thap1 floxed allele (bottom right). (B) Thap1 expression based on lacZ co-expression in a P50 Thap1+/− mouse brain (scale bar: 1 mm) and panel below of STR and CB (scale bar: 250 μm). (C) Time course of Thap1 based on lacZ co-expression in Thap1+/− mouse brains starting at P0 (scale bar: 1 mm). (D) Quantitative RT-PCR of cerebellar, cortical, striatal and liver (control) tissue for Thap1 mRNA in wild-type and Thap1 cKO mice normalized to Gapdh (n = 4, two-way ANOVA, F(3,20) = 5.059; **P < 0.01).