Figure 4.

EAP1 binding to the GnRH promoter increases at puberty of female rhesus monkeys. An increased association of EAP1 to the rhesus GnRH1 promoter region in the MBH of female monkeys between the EJ and LJ periods is demonstrated. No change in EAP1 binding is detected in intron 2 of the GnRH gene. Inp means Input DNA and bGal means ChIP performed using an antibody to beta-galactosidase (a protein not present in the brain), serves as a negative control. Experiment was performed once, due to the limitation of availability of primate tissue.