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. 2019 Mar 25;374(1772):20180089. doi: 10.1098/rstb.2018.0089

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© 2019 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 4. — Characterizing non-canonical PAMs. (a) The frequency of a canonical GA PAM ± 3 nt from a non-canonical GG PAM across all data sets. (b) Tenfold dilutions of ICP1 engineered to contain a spacer that targets PLE 1 with a non-canonical PAM spotted on V. cholerae PLE (−) or PLE 1 lawns showing the ability of different phage strains to form plaques (dark spots, zones of killing) (left). Sequence context (right) of the region adjacent to the PAM. The protospacer is boxed in purple and PAM is boxed in pink. The consensus canonical PAM GA is bolded and underlined.