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Representative flow cytometric multicolour gating strategy used to analyse number of MPs. (A) Location of 1 µm beads used to gate MP events according to size. (B) Representative of MPs events identified according to size (<1 µm). (C) Annexin V positive events (MPs). (D) PMPs (Annexin V+ CD31+ CD42b+) and ECMPs (Annexin V+ CD31+ CD42b-) populations were identified. (E, F) VEGFR2-expressing PMPs (Annexin V+ CD31+ CD42b+ CD309+) and ECMPs (Annexin V+ CD31+ CD42b- CD309+) were selected.
