Table 2.
Biomarker measurement.
| # | Year | Author | Subjects (n) | Sample origin∗ | Gene marker∗∗ | Sample preparation | Method |
|---|---|---|---|---|---|---|---|
| 1 | 2010 | Taoudi Benchekroun et al. | 162 | HB | EGFR (U) | Human OC biopsy formalin fixed and paraffin-embedded | FISH |
| 2 | 2012 | Jung et al. | 17 | TB | 134 different miRNAs (see image 1) | Cell culture and transfection of oral cancer cells and normal cell biopsy | mirVana™, microarray gene expression, qRT-PCR |
| 3 | 2013 | Minakawa et al. | 106 | TB | KIFGA (U) | Immortalized human OSCC-derived cell lines obtained from the tissue bank. Human biopsy fixed in 20% buffered formaldehyde solution | qRT-PCR |
| 4 | 2015 | Luo et al. | 121 | HB | OPN (osteopontin) | Human OC biopsy formalin fixed and paraffin-embedded | Western blot |
| 5 | 2014 | Su et al. | 7 | HB | DEPDC1B (U) | Human biopsy | Immunoprecipitation, Northern blot, Western blot |
| 6 | 2011 | Cao et al. | 76 | TB | EZM2(D) | Human biopsy sample paraffin included and sectioned. Colored with H&E | Western blot |
| 7 | 2009 | Saintigny et al. | 162 | HB | deltaNp63 (U), EIC (U), podoplanin (U) | Human OC biopsy formalin fixed and paraffin-embedded | Cell membrane immunoreactivity, microscope |
| 8 | 2011 | Saintigny | 162 | HB | Has-miR-101 (D), deltaNp63 (U), P63 (U), DNMT3B (U) | Whole biopsy including both the epithelial cells and the underlying stroma | Microarray gene expression |
∗Type of sample: HU: human biopsy; TB: tissue bank sample. ∗∗Type of altered gene regulation: D: downregulation, diminution; U: upregulation, augmentation.