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. 2019 Mar 25;2019:1897316. doi: 10.1155/2019/1897316

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Copyright © 2019 Jun Li et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Pretreatment with cholecalciferol alleviates renal ER stress during I/R-induced AKI. In the I/R and Chol + I/R groups, mice were conducted with ischemia/reperfusion surgery. In the Chol and Chol + I/R groups, mice were orally administered with three doses of cholecalciferol (25 μg/kg) at 48, 24, and 1 h before I/R surgery. Kidney samples were collected with a 4 h period of reperfusion after ischemia surgery. (a, b) Renal GRP78 was measured using immunoblots. (a) A representative gel for GRP78 and β-actin was shown. (b) GRP78/β-actin. (c) Renal grp78 mRNA was measured using real-time RT-PCR. (d, e) Renal GRP78 was analyzed by immunohistochemistry. (d) Representative photomicrographs of renal specimens from different groups are presented. Original magnification 400x. Renal GRP78 was mainly distributed in cortical tubular epithelial cells. (e) GRP78⁺ cells were analyzed. (f–i) Renal p-IRE1α, p-eIF2α, and CHOP were measured using immunoblots. (f) A representative gel for p-IRE1α, p-eIF2α, CHOP, and β-actin was shown. (g) p-IRE1α/β-actin. (h) p-eIF2α/β-actin. (i) CHOP/β-actin. All data were expressed as means ± SEM (n = 6). ^∗∗P < 0.01.