Figure 4.

Effects of ERK inhibition on TTX-R resurgent currents in medium DRG neurons. Representative TTX-R, slow resurgent currents were recorded from medium DRG neurons (a–d). The resurgent currents were elicited by the same voltage protocol as described in Figure 1. ERK inhibitor U0126 (10 µM) and its inactive control U0124 (10 µM) were pretreated for 15 min in culture medium and were maintained in the recording chamber. (a and b) In the presence of U0124, the TTX-R resurgent currents were enhanced by inflammatory mediators (IMs). (c and d) U0126 completely prevented the enhancing effects of inflammatory mediators on the TTX-R resurgent currents. Note that U0126 also reduced TTX-R resurgent currents compared to U0124 insignificantly (P < 0.1). (e) The data were presented as mean ± standard error of the mean. Student’s t-test was used to compare the difference, *P < 0.05 (vs. U0124).