Figure 7.

Effects of PKC inhibition on TTX-R resurgent currents in small DRG neurons. Representative TTX-R, slow resurgent currents were recorded from small DRG neurons (a–d). The resurgent currents were elicited by the same voltage protocol as described in Figure 1. PKC inhibitor BIM I (1 µM) and control DMSO (1:1000) were pretreated for 15 min in culture medium and were maintained in the recording chamber. (a and b) In DMSO control condition, the TTX-R resurgent currents were enhanced by inflammatory mediators (IMs). (c and d) BIM I completely prevented the enhancing effects of inflammatory mediators on the TTX-R resurgent currents. (e) The data were presented as mean ± standard error of the mean. Student’s t-test was used to compare the difference, **P < 0.01 (vs. DMSO). DMSO: dimethyl sulfoxide; BIM I: bisindolylmaleimide I.