EE experience enhances LTP in CA1-RARα KO mice by stimulating AMPAR protein synthesis. (A) SC-CA1 LTP induced by the voltage pulse protocol in EE-experienced WT and CA1-RARα KO mice (two-tailed Mann–Whitney U test: **P < 0.01). n/N, number of neurons/number of animals. (B) Immunoblot analysis of total GluA1 and GluA2 expression in home cage (HC) WT and CaMKII-Cre RARα KO CA1 (two-tailed Student’s t test: GluA1: t = 3.572, df = 10, **P < 0.01; GluA2: t = 0.7464, df = 9, P > 0.4). (C) Immunoblot analysis of total GluA1 and GluA2 expression in HC- and EE-experienced WT CA1 (two-tailed Student’s t test: GluA1: t = 2.079, df = 10, P = 0.064; GluA2: t = 2.096, df = 10, P = 0.063). (D) Immunoblot analysis of total GluA1 and GluA2 expression in HC- and EE-experienced CaMKII-Cre RARα KO CA1 (two-tailed Student’s t test: GluA1: t = 6.539, df = 8, ***P < 0.001; GluA2: t = 4.681, df = 8, **P < 0.01). All graphs represent mean ± SEM.