Fig. 3.

Effects of TPPU on behavioral abnormalities and the reduction of PV-IR and GAD₆₇-IR in the mPFC of adult offspring after MIA. (A) Schedule of treatment and brain collection. Saline or poly(I:C) (5 mg/kg/d for 6 d) was administered i.p. to pregnant mice. Vehicle or TPPU (15 mg/L) in drinking water was administered to juvenile offspring from P28 to P56. Subsequently, all mice received normal water. Locomotion and NORT were performed when the mice were 10 wk old. Brain for immunohistochemistry was collected at P72. (B) Locomotion test. (C) NORT. (D) PV immunohistochemistry. (E) GAD₆₇ immunohistochemistry. *P < 0.05, **P < 0.01, and ***P < 0.001 compared with poly(I:C) + vehicle group. IL: infralimbic of medial prefrontal cortex. N.S, not significant. PrL, prelimbic of medial prefrontal cortex. Detailed statistical analysis data are in SI Appendix, Table S6.