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. 2019 Mar 8;116(14):7027–7032. doi: 10.1073/pnas.1819796116

Fig. 2.

Fig. 2.

The influence of mexT, pqsA, and pqsE mutations on RhlR activity in the P. aeruginosa LasR-PsdR mutant. (A) Growth of P. aeruginosa mutants in casein broth. Bacteria were tagged with mCherry, and fluorescence (RFU) was used as a proxy for growth, as described in Materials and Methods. (B) RhlR activity in the LasR-PsdR mutant and mutants derived from this strain, measured as GFP fluorescence (RFU). All strains contained the PrhlA-gfp fusion plasmid pProbe-GT-PrhlAgfp. (C) RhlR activity in strain PAO1 compared with RhlR activity in the LasR-PsdR-MexT mutant, measured as in B. (D) Relative concentrations of C4-HSL produced by the WT and mutants after 18 h. (E) Image of culture tubes after 2 d of growth of the indicated strains in casein broth. The LasR-PsdR-MexT mutant culture is blue green as a result of pyocyanin production. Data in A and D are means of duplicate experiments, and data in B and C are means of triplicate experiments. In all panels, error bars represent the SEM.