Table 1.
Variant* | mexT mutation | MexT change | Other affected genes |
LasR mutants | |||
LasR-1 | T353A | L118Q | None |
LasR-2 | A122G | S41G | None |
LasR-3 | Δ(2793006-2812975) | ΔmexT | PA2475, dsbG, PA2477-PA2490, mexS, mexE, mexF |
LasR130-1 | Δ(2808103-2808997) | Truncation | NA† |
LasR130-2 | Δ(A768) | Frameshift | NA† |
LasR130-3 | A559C | T560P | NA† |
LasR131-1 | A55C | T19P | NA† |
LasR131-2 | G31C, C478G | V11L, L160V | NA† |
LasR131-3 | NP‡ | NP‡ | NA† |
LasI mutants | |||
LasI-1 | A55C | T19P | psdR |
LasI-2 | A55C | T19P | psdR |
LasI-3 | A55C | T19P | psdR, fleQ |
Variants LasR-1–3 were isolated from casein broth inoculated with the LasR mutant only, LasR130-1-3 and LasR131-1-3 were isolated from casein broth coinoculated with the LasR mutant and clinical P. aeruginosa isolates E130 or E131, and LasI-1–3 were isolated from casein broth inoculated with the LasI mutant. Further details about the variants can be found in SI Appendix, Table S1.
NA, other genes were not analyzed in these isolates.
NP, no product. We could not amplify mexT for sequencing.