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The E80 protease-deficient freeloaders show reduced levels of C4-HSL and 2-alkylquinolone production. (A) Relative C4-HSL levels in cultures of strain E80, E80 freeloaders (1–6), and E80 freeloaders 1 and 2 complemented with a chromosomal copy of pqsR (1-C and 2-C). Values are normalized to E80 levels. (B) Concentrations of PQS (black) and HHQ (white) produced in the indicated strains. For both A and B, data are the means of four replicates ± SD.
