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. 2019 Mar 14;116(14):7062–7070. doi: 10.1073/pnas.1900144116

Fig. 1.

Fig. 1.

F. tularensis OAg is susceptible to acid hydrolysis, but its structural identity is conserved. (A) Silver staining and immunoblot analysis of LPS from F. tularensis (Ft) LVS using an OAg-specific mAb (mAb2034). RU, repeating unit. (B) SEC profiles of HMW and LMW OAgs extracted from F. tularensis LVS. A profile run on a Superose 6 10/300 GL column at 0.5 mL⋅min−1 with 1× PBS (pH 7.4) is shown. The average molecular weight was calculated with a dextran calibration curve. mAU, milli-absorbance unit. (C) 1H NMR spectrum of HMW and LMW OAgs extracted from F. tularensis LVS.