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. 2019 Mar 14;116(14):7062–7070. doi: 10.1073/pnas.1900144116

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Copyright © 2019 the Author(s). Published by PNAS.

This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).

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Fig. 5. — Size of the OAg affects relative affinity to LPS-specific antibodies. (A) Anti-OAg IgG ELISA units to the F. tularensis ∆wzz1/wzz2^Fn LPS coating agent measured at day 49. Dots represent individual animals. Horizontal bars represent mean ± SD values. (B) F. tularensis (Ft) OAg competitive ELISA. Recognition of Ft OAg-specific polyclonal antibody by UV-killed Ft-coated ELISA plates in the presence of LMW (orange), HMW (green), or VHMW (red) OAg as a soluble competitor is shown. The inhibition percentage is calculated in relation to the ELISA signal (A₄₀₅) with no competition. Data points represent competition percentage values at indicated inhibitor concentrations. Each data point is the mean of duplicate determinations from a representative experiment. Horizontal bars represent mean ± SD values. *P ≤ 0.05.