Table 3.

Primers and plasmids used in study

Primer/plasmid	Sequence/description	Reference
Primers
NHL207	5′-gctgcaggaattcgatATCTCCAGTAGAAGCTACG	Upstream region forward wzz1 deletion
NHL208	5′-gcacaatattTAGCTTGGTTTAAATAACCAAG	Upstream region reverse, wzz1 deletion
NHL209	5′-ccaagctaAATATTGTGCAAAGACAGATTAAATTATG	Downstream region forward, wzz1 deletion
NHL210	5′-ggtatcgataagcttgatGATGATCTATGGTTGCTGAAG	Downstream region reverse, wzz1 deletion
NHL86	5′-atcaagcttatcgataccg	pMP812 forward primer
NHL87	5′-atcgaattcctgcagccc	pMP812 reverse primer
NHL66	5′-ctaagaaattctaataatgatTATACCCTTCAAGCTTTGAAAAATAAAC	groEL promoter forward, wzz1 expression
NHL203	5′-tcagccatAACAATCTTACTCCTTTGTTAAATTATTTTTG	groEL promoter reverse, wzz1 expression
NHL204	5′-taagattgttATGGCTGAAATTAAAAATGATGAATATATAG	FTL_0589 forward, wzz1 expression
NHL208	5′gcattatccaatgcagatttagtggtgatggtgatgatgTTGTTTAAGATTTCTATATTCAGTAATC	FTL_0589 reverse, wzz1 expression
NHL69	5′-atctgcattggataatgcattg	pMP633 forward primer
NHL70	5′-atcattattagaatttcttagaaatatgaa	pMP633 reverse primer
Plasmids
pMP812	KmR, sacB suicide vector	(62)
pMP633	HygR, E. coli-F. tularensis shuttle vector	(63)
pNA45	pMP812-derived F. tularensis ∆wzz1 (FTL_0589) deletion plasmid	This work
pNA49	pMP633-derived F. tularensis wzz1 (FTL_0589) expression under the control of groEL promoter	This work